Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats

Authors

  • Amir Mahdi Khamaneh Department of molecular medicine, School of advanced medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran
  • Hadi Ebrahimi Tuberculosis and lung diseases research center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Maryam Fallahi Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Rana Keyhanmanesh Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Saeideh Saadat Department of Physiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Abstract:

Objectives: In previous studies the therapeutic effects of Nigella sativa have been demonstrated on asthmatic animals. In the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs of ovalbumin sensitized rat in order to elicit its mechanism. Materials and methods: Forty rats were randomly grouped in 4 groups; control (C), sensitized (S), sensitized pretreated groups with thymoquinone (3 mg/kg i.p., S+TQ) and alpha-hederin (0.02 mg/kg i.p., S+AH). Levels of IL-13 mRNA and miRNA-126 in lung tissue and its pathological changes in each group were assessed. Results: Elevated levels of miRNA-126, IL-13 mRNA and pathological changes were observed in the sensitized group compared to the control group (pConclusion: The results suggested that alpha-hederin had preventive effect on sensitized rats like thymoquinone. It may intervene in miRNA-126 expression, which consequently could interfere with IL-13 secretion pathway leading to a reduction in inflammatory responses.

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effect of alpha-hederin, the active constituent of nigella sativa, on mirna-126, il-13 mrna levels and inflammation of lungs in ovalbumin-sensitized male rats

objectives: in previous studies the therapeutic effects of nigella sativa have been demonstrated on asthmatic animals. in the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs of ovalbumin sensitized rat in order to elicit its mechanism. materials and methods: forty rats ...

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Journal title

volume 6  issue 1

pages  77- 85

publication date 2016-01-01

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